gastric cancer cell lines mkn45  (Procell Inc)

Journal: Materials Today Bio

Article Title: FAPI-engineered nanoprobe induces dual-cell ferroptosis therapy and enables light-up magnetic resonance imaging for gastric cancer peritoneal metastasis

doi: 10.1016/j.mtbio.2025.102261

Figure Lengend Snippet: Assessment of cytotoxicity and proliferation inhibition of CDDP2@MSPION@GP3/FAPI on MKN45 cells and CAFs. A–B) The viabilities of MKN45 cells (A) and CAFs (B) treated with PBS (I), MSPION (II), CDDP (III), CDDP2@MSPION@GP3 (IV) or CDDP2@MSPION@GP3/FAPI (V) for 24 h. C) The viabilities of NFs and CAFs treated with CDDP2@MSPION@GP3/FAPI for 24 h. D–F) Fluorescence images (D) and flow cytometry analysis (E–F) of MKN45 cells from different treatment groups after staining with Calcein-AM/PI or 7-AAD. G–I) Fluorescence images (G) and flow cytometry analysis (H–I) of CAFs from different treatment groups after staining with Calcein-AM/PI or 7-AAD. J–L) Fluorescence images (J) and flow cytometry analysis (K–L) of MKN45 cells from different treatment groups after staining with EdU. M−O) Fluorescence images (M) and flow cytometry analysis (N–O) of CAFs from different treatment groups after staining with EdU. Scale bar: 100 μm ∗ P < 0.05. ∗∗ P < 0.01. ∗∗∗ P < 0.001. ∗∗∗∗ P < 0.0001.

Article Snippet: The human gastric cancer cell line MKN45 was purchased from Procell Life Science & Technology Co., Ltd. (Cat. No. CL-0292).

Techniques: Inhibition, Fluorescence, Flow Cytometry, Staining

Journal: Materials Today Bio

Article Title: FAPI-engineered nanoprobe induces dual-cell ferroptosis therapy and enables light-up magnetic resonance imaging for gastric cancer peritoneal metastasis

doi: 10.1016/j.mtbio.2025.102261

Figure Lengend Snippet: Evaluation of ferroptosis characteristics induced by CDDP2@MSPION@GP3/FAPI in MKN45 cells and CAFs. A–B) Fluorescence distribution (A) and quantification of ROS levels (B) in MKN45 cells treated with PBS (I), MSPION (II), CDDP (III), CDDP2@MSPION@GP3 (IV) or CDDP2@MSPION@GP3/FAPI (V) for 24 h, detected by DCFH-DA staining. C–D) Fluorescence distribution (C) and quantification of ROS levels (D) in CAFs from different treatment groups, detected by DCFH-DA staining. E–G) GPX4 expression in MKN45 cells and CAFs from different treatment groups as determined by Western blot (E), with corresponding statistical analysis (F–G). H–J) CLSM images (H) and corresponding flow cytometry analysis (I–J) of MKN45 cells from different treatment groups, stained with JC-1 to assess mitochondrial membrane potential. Red fluorescence indicates JC-1 aggregates in healthy mitochondria, while green fluorescence represents JC-1 monomers in depolarized (damaged) mitochondria. K–M) CLSM images (K) and corresponding flow cytometry analysis (L–M) of JC-1 stained CAFs from different treatment groups. N-P) CLSM images (N), flow cytometry fluorescence distributions (O), and the quantitative analysis (P) of MKN45 cells after treatments of groups I–V and staining with DAPI and C11-BODIPY. Red fluorescence: reduced form for the decreased LPO. Green fluorescence: oxidized form for the increased LPO. Q–S) CLSM images (Q), flow cytometry fluorescence distributions (R), and the quantitative analysis (S) of CAFs after treatments of groups I–V and staining with DAPI and C11-BODIPY 581/591. Scale bar: 100 μm ∗ P < 0.05. ∗∗ P < 0.01. ∗∗∗ P < 0.001. ∗∗∗∗ P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The human gastric cancer cell line MKN45 was purchased from Procell Life Science & Technology Co., Ltd. (Cat. No. CL-0292).

Techniques: Fluorescence, Staining, Expressing, Western Blot, Flow Cytometry, Membrane

Journal: Materials Today Bio

Article Title: FAPI-engineered nanoprobe induces dual-cell ferroptosis therapy and enables light-up magnetic resonance imaging for gastric cancer peritoneal metastasis

doi: 10.1016/j.mtbio.2025.102261

Figure Lengend Snippet: Inhibition of metastasis by modulating the crosstalk between MKN45 cells and CAFs using CDDP2@MSPION@GP3/FAPI. (A) Schematic illustration of the experimental design to evaluate the anti-metastatic effect of CDDP2@MSPION@GP3/FAPI by assessing its impact on MKN45 cells under different CM. (B–C) Representative images of colony formation (B) and wound healing (C) assays of MKN45 cells treated with: PBS (I), NFs CM (II), CAFs CM (III) or CAFs CM after treatment with MSPION (IV), CDDP (V), CDDP2@MSPION@GP3 (VI), or CDDP2@MSPION@GP3/FAPI (VII). (D) Schematic illustration of the Transwell co-culture assay of MKN45 cells and CAFs. (E) Representative optical images of migration and invasion assays of MKN45 cells co-cultured with NFs (II) or CAFs (III), with the CAFs group treated with MSPION (IV), CDDP (V), CDDP2@MSPION@GP3 (VI), or CDDP2@MSPION@GP3/FAPI (VII). (F) Quantitative analysis of the colony formation assay. (G) Quantitative analysis of the wound healing assay. (H) Quantitative analysis of IL-6 levels by ELISA. (I) Quantitative analysis of the migration assay. (J) Quantitative analysis of the invasion assay. Scale bar: 200 μm ∗ P < 0.05. ∗∗ P < 0.01. ∗∗∗ P < 0.001. ∗∗∗∗ P < 0.0001.

Article Snippet: The human gastric cancer cell line MKN45 was purchased from Procell Life Science & Technology Co., Ltd. (Cat. No. CL-0292).

Techniques: Inhibition, Co-culture Assay, Migration, Cell Culture, Colony Assay, Wound Healing Assay, Enzyme-linked Immunosorbent Assay, Invasion Assay

Journal: Materials Today Bio

Article Title: FAPI-engineered nanoprobe induces dual-cell ferroptosis therapy and enables light-up magnetic resonance imaging for gastric cancer peritoneal metastasis

doi: 10.1016/j.mtbio.2025.102261

Figure Lengend Snippet: Evaluation of the anti-tumor efficacy of CDDP2@MSPION@GP3/FAPI in the subcutaneous tumor model. A) Schematic illustration of the establishment and subsequent treatment of a GC subcutaneous tumor model constructed with MKN45 cells and CAFs. B) Relative body weight curves of tumor-bearing mice. C) Relative tumor volume. D) Photographs of tumors isolated from mice in different groups after 14 days of treatment. E) Average tumor weight. F) Representative microscope images of tumor tissue sections stained with H&E, GPX4, Ki-67, α- SMA and TUNEL. G–J) Quantitative analysis of the results shown in (F). Scale bar: 100 μm ∗ P < 0.05. ∗∗ P < 0.01. ∗∗∗ P < 0.001. ∗∗∗∗ P < 0.0001.

Article Snippet: The human gastric cancer cell line MKN45 was purchased from Procell Life Science & Technology Co., Ltd. (Cat. No. CL-0292).

Techniques: Construct, Isolation, Microscopy, Staining, TUNEL Assay

Journal: Materials Today Bio

Article Title: FAPI-engineered nanoprobe induces dual-cell ferroptosis therapy and enables light-up magnetic resonance imaging for gastric cancer peritoneal metastasis

doi: 10.1016/j.mtbio.2025.102261

Figure Lengend Snippet: Evaluation of the anti-tumor efficacy of CDDP2@MSPION@GP3/FAPI in the PM model. A) Schematic illustration of the establishment and subsequent treatment of the PM model constructed with MKN45 cells and CAFs. B) Relative body weight curves of tumor-bearing mice. C) Photographs of peritoneal metastases in mice from different treatment groups. D) Number of peritoneal nodules. E) Average weight of peritoneal nodules. F) Representative microscope images of tumor tissue sections stained with H&E, GPX4, Ki-67, α- SMA and TUNEL. G–J) Quantitative analysis of the results shown in (F). Scale bar: 100 μm ∗ P < 0.05. ∗∗ P < 0.01. ∗∗∗ P < 0.001. ∗∗∗∗ P < 0.0001.

Article Snippet: The human gastric cancer cell line MKN45 was purchased from Procell Life Science & Technology Co., Ltd. (Cat. No. CL-0292).

Techniques: Construct, Microscopy, Staining, TUNEL Assay